![]() ![]() Finally, P53mRNA and phospho-P53 protein levels were further investigated in order to explore a possible explanation. The distribution of cell cycle and the change of apoptosis rates were detected by a flow cytometric method (FCM). Cellular survival rate were investigated by MTT growth inhibition assay, DNA damage levels were investigated by comet assay and RAD51 staining. Second, all cells were treated with cisplatin. First, cDNA clones expressing different genotypes of the polymorphism was transfected to an ERCC 2/XPD defective CHO cell line (UV5). The aim of the present study was to investigate whether ERCC 2/XPD Lys751Gln ( rs 13181) polymorphism is causally related to DNA repair capacity of platinum-induced DNA damage. Single nucleotide polymorphisms in ERCC 2/XPD have been found to be associated with platinum resistance. The platinum-induced DNA damage is recognized and repaired by the nucleotide excision repair (NER) system of which ERCC 2/XPD is a critical enzyme. Platinum-based treatment causes Pt-DNA adducts which lead to cell death. Zhang, Guopei Guan, Yangyang Zhao, Yuejiao van der Straaten, Tahar Xiao, Sha Xue, Ping Zhu, Guolian Liu, Qiufang Cai, Yuan Jin, Cuihong Yang, Jinghua Wu, Shengwen Lu, Xiaobo ERCC 2/XPD Lys751Gln alter DNA repair efficiency of platinum-induced DNA damage through P53 pathway.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |